The April 2024 issue of IEEE Spectrum is here!

Close bar

Building 3D Nano Structures with RNA Resistant to Enzymes

Researchers make enzyme-resistant RNA that could enable bottom up assembly of 3D nanostructures

2 min read

Building 3D Nano Structures with RNA Resistant to Enzymes

The field of so-called RNA nanotechnology has been around for awhile, but it has had difficulty advancing as much as DNA nanotechnology despite its more flexible capabilities because of an enzyme known as RNase that eats RNA within minutes, making it extremely difficult to build anything with RNA without it being cut up into pieces in short order


But Peixuan Guo and his colleagues at the University of Cincinnati have developed a method by which they replace a chemical group in the RNA making it become resistant to the RNase enzyme. Guo has been spearheading research with RNA and with this latest paper published in the American Chemical Society’s journal Nano he and his team may have established a way to take advantage of this macromolecule for building nanostructures from the bottom up.

Guo and his team focused their research on the ribose rings that along with alternating phosphate groups make up the backbone of RNA. By replacing one of the rings the researchers made it so the RNase could not attach itself to the RNA.

The advantages of RNA over DNA in bottom-up fabrication are that it can be manipulated like DNA “while possessing noncanonical base-pairing, versatile function, and catalytic activity similar to proteins.”

The Foresight Institute’s blog Nanodot has excellent coverage of this development that was written by Jim Lewis, who, according to his article, has spent most of his research career working with RNA.

Guo will be using the RNA in “gearing a powerful nanomotor that packages viral DNA into the protein shells of a bacterial virus named phi29.” 

Lewis’ closing sentence in his Foresight report sums it up eloquently: “It will be interesting to watch over the next several years if this variety of 3D structures leads to useful structures and devices for the development of molecular machine systems and ultimately productive nanosystems.”

The Conversation (0)